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cd-1 timed pregnant female mice embryonic day 16 (e16)  (Charles River Laboratories)

 
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    Structured Review

    Charles River Laboratories cd-1 timed pregnant female mice embryonic day 16 (e16)
    (A) <t>E16</t> SMG were subjected to enzymatic treatment using collagenase/hyaluronidase/dispase (C/H/D) to generate lobules followed by incubation and trituration to separate the epithelial clusters (ECs) and mesenchyme cells through three sequential gravity sedimentation steps (Basic Protocol 1). (B) Brightfield images showed the morphology of the gland before enzymatic treatment, and after enzyme treatment to isolate lobules. After processing and gravity sedimentation, the ECs are enriched in the gravity pellet, and the mesenchyme cells are enriched in the gravity supernatant. (C) ICC (as described in Support Protocol 5) and confocal imaging revealed that AQP5 is apically localized in the acini in pieces of the whole gland before enzymatic digestion, in lobules, and in epithelial clusters from the gravity pellet prepared as time zero samples (as described in Support Protocol 3). Gravity supernatant enriched for mesenchymal cells includes cells that express vimentin. Markers: EpCAM (epithelial, green), AQP5 (proacinar/acinar, red), and vimentin (mesenchyme, cyan) with DAPI staining (nuclei, blue). Individual channels are shown at right from boxed areas, as reported in Hosseini et al., 2018. Scale bar = 200 μm for (B) and 50 μm for (C). N = 3 experiments. Mes = mesenchymal cells, EC = epithelial clusters, Supe = supernatant.
    Cd 1 Timed Pregnant Female Mice Embryonic Day 16 (E16), supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd-1+timed+pregnant+female+mice+embryonic+day+16+(e16)/c57bl+6+mice/pmc06948183-50-95-103
    Average 90 stars, based on 1 article reviews
    cd-1 timed pregnant female mice embryonic day 16 (e16) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Generating Embryonic Salivary Gland Organoids"

    Article Title: Generating Embryonic Salivary Gland Organoids

    Journal: Current protocols in cell biology

    doi: 10.1002/cpcb.76

    (A) E16 SMG were subjected to enzymatic treatment using collagenase/hyaluronidase/dispase (C/H/D) to generate lobules followed by incubation and trituration to separate the epithelial clusters (ECs) and mesenchyme cells through three sequential gravity sedimentation steps (Basic Protocol 1). (B) Brightfield images showed the morphology of the gland before enzymatic treatment, and after enzyme treatment to isolate lobules. After processing and gravity sedimentation, the ECs are enriched in the gravity pellet, and the mesenchyme cells are enriched in the gravity supernatant. (C) ICC (as described in Support Protocol 5) and confocal imaging revealed that AQP5 is apically localized in the acini in pieces of the whole gland before enzymatic digestion, in lobules, and in epithelial clusters from the gravity pellet prepared as time zero samples (as described in Support Protocol 3). Gravity supernatant enriched for mesenchymal cells includes cells that express vimentin. Markers: EpCAM (epithelial, green), AQP5 (proacinar/acinar, red), and vimentin (mesenchyme, cyan) with DAPI staining (nuclei, blue). Individual channels are shown at right from boxed areas, as reported in Hosseini et al., 2018. Scale bar = 200 μm for (B) and 50 μm for (C). N = 3 experiments. Mes = mesenchymal cells, EC = epithelial clusters, Supe = supernatant.
    Figure Legend Snippet: (A) E16 SMG were subjected to enzymatic treatment using collagenase/hyaluronidase/dispase (C/H/D) to generate lobules followed by incubation and trituration to separate the epithelial clusters (ECs) and mesenchyme cells through three sequential gravity sedimentation steps (Basic Protocol 1). (B) Brightfield images showed the morphology of the gland before enzymatic treatment, and after enzyme treatment to isolate lobules. After processing and gravity sedimentation, the ECs are enriched in the gravity pellet, and the mesenchyme cells are enriched in the gravity supernatant. (C) ICC (as described in Support Protocol 5) and confocal imaging revealed that AQP5 is apically localized in the acini in pieces of the whole gland before enzymatic digestion, in lobules, and in epithelial clusters from the gravity pellet prepared as time zero samples (as described in Support Protocol 3). Gravity supernatant enriched for mesenchymal cells includes cells that express vimentin. Markers: EpCAM (epithelial, green), AQP5 (proacinar/acinar, red), and vimentin (mesenchyme, cyan) with DAPI staining (nuclei, blue). Individual channels are shown at right from boxed areas, as reported in Hosseini et al., 2018. Scale bar = 200 μm for (B) and 50 μm for (C). N = 3 experiments. Mes = mesenchymal cells, EC = epithelial clusters, Supe = supernatant.

    Techniques Used: Incubation, Sedimentation, Imaging, Staining



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    cd-1 timed pregnant female mice embryonic day 16 (e16)  (Charles River Laboratories)
    90
    Charles River Laboratories cd-1 timed pregnant female mice embryonic day 16 (e16)
    (A) <t>E16</t> SMG were subjected to enzymatic treatment using collagenase/hyaluronidase/dispase (C/H/D) to generate lobules followed by incubation and trituration to separate the epithelial clusters (ECs) and mesenchyme cells through three sequential gravity sedimentation steps (Basic Protocol 1). (B) Brightfield images showed the morphology of the gland before enzymatic treatment, and after enzyme treatment to isolate lobules. After processing and gravity sedimentation, the ECs are enriched in the gravity pellet, and the mesenchyme cells are enriched in the gravity supernatant. (C) ICC (as described in Support Protocol 5) and confocal imaging revealed that AQP5 is apically localized in the acini in pieces of the whole gland before enzymatic digestion, in lobules, and in epithelial clusters from the gravity pellet prepared as time zero samples (as described in Support Protocol 3). Gravity supernatant enriched for mesenchymal cells includes cells that express vimentin. Markers: EpCAM (epithelial, green), AQP5 (proacinar/acinar, red), and vimentin (mesenchyme, cyan) with DAPI staining (nuclei, blue). Individual channels are shown at right from boxed areas, as reported in Hosseini et al., 2018. Scale bar = 200 μm for (B) and 50 μm for (C). N = 3 experiments. Mes = mesenchymal cells, EC = epithelial clusters, Supe = supernatant.
    Cd 1 Timed Pregnant Female Mice Embryonic Day 16 (E16), supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd-1+timed+pregnant+female+mice+embryonic+day+16+(e16)/c57bl+6+mice/pmc06948183-50-95-103
    Average 90 stars, based on 1 article reviews
    cd-1 timed pregnant female mice embryonic day 16 (e16) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) E16 SMG were subjected to enzymatic treatment using collagenase/hyaluronidase/dispase (C/H/D) to generate lobules followed by incubation and trituration to separate the epithelial clusters (ECs) and mesenchyme cells through three sequential gravity sedimentation steps (Basic Protocol 1). (B) Brightfield images showed the morphology of the gland before enzymatic treatment, and after enzyme treatment to isolate lobules. After processing and gravity sedimentation, the ECs are enriched in the gravity pellet, and the mesenchyme cells are enriched in the gravity supernatant. (C) ICC (as described in Support Protocol 5) and confocal imaging revealed that AQP5 is apically localized in the acini in pieces of the whole gland before enzymatic digestion, in lobules, and in epithelial clusters from the gravity pellet prepared as time zero samples (as described in Support Protocol 3). Gravity supernatant enriched for mesenchymal cells includes cells that express vimentin. Markers: EpCAM (epithelial, green), AQP5 (proacinar/acinar, red), and vimentin (mesenchyme, cyan) with DAPI staining (nuclei, blue). Individual channels are shown at right from boxed areas, as reported in Hosseini et al., 2018. Scale bar = 200 μm for (B) and 50 μm for (C). N = 3 experiments. Mes = mesenchymal cells, EC = epithelial clusters, Supe = supernatant.

    Journal: Current protocols in cell biology

    Article Title: Generating Embryonic Salivary Gland Organoids

    doi: 10.1002/cpcb.76

    Figure Lengend Snippet: (A) E16 SMG were subjected to enzymatic treatment using collagenase/hyaluronidase/dispase (C/H/D) to generate lobules followed by incubation and trituration to separate the epithelial clusters (ECs) and mesenchyme cells through three sequential gravity sedimentation steps (Basic Protocol 1). (B) Brightfield images showed the morphology of the gland before enzymatic treatment, and after enzyme treatment to isolate lobules. After processing and gravity sedimentation, the ECs are enriched in the gravity pellet, and the mesenchyme cells are enriched in the gravity supernatant. (C) ICC (as described in Support Protocol 5) and confocal imaging revealed that AQP5 is apically localized in the acini in pieces of the whole gland before enzymatic digestion, in lobules, and in epithelial clusters from the gravity pellet prepared as time zero samples (as described in Support Protocol 3). Gravity supernatant enriched for mesenchymal cells includes cells that express vimentin. Markers: EpCAM (epithelial, green), AQP5 (proacinar/acinar, red), and vimentin (mesenchyme, cyan) with DAPI staining (nuclei, blue). Individual channels are shown at right from boxed areas, as reported in Hosseini et al., 2018. Scale bar = 200 μm for (B) and 50 μm for (C). N = 3 experiments. Mes = mesenchymal cells, EC = epithelial clusters, Supe = supernatant.

    Article Snippet: Materials Dissecting microscope with transmitted light base and overhead light Inverted Tissue Culture Grade Microscope equipped with a digital camera such as a Nikon Eclipse TS100 microscope equipped with a Canon EOS 450D digital camera at 4x (Plan 4x /0.10 NA) or 10x (10x Ph1 ADL/0.25 NA) Standard tissue culture incubator, humidified at 37°C with 5% CO 2 Standard tissue culture benchtop centrifuge Standard water bath Standard serological pipets and pipetmen with standard tips Standard class II biological safety cabinet Hank’s balanced salt solution (HBSS) (ThermoFisher Scientific) CD-1 timed pregnant female mice embryonic day 16 (E16) (day 0 is day of plug discovery) (Charles River Laboratories) Two fine forceps: dumont #5; inox alloy, 0.05mm X 0.02mm (Fine Science Tools) Collagenase/hyaluronidase (Stem Cell Technologies) Dispase II (Gibco) 1XPBS (ThermoFisher Scientific) DMEM:F12, no phenol red (ThermoFisher Scientific) (21041025) Penicillin 10,000 U/mL / Streptomycin 10,000 μg/ml (ThermoFisher Scientific) Fetal Bovine Serum (FBS) (ThermoFisher Scientific) 35 mm dishes (Corning) 15 ml conical tubes (Corning) Collect submandibular glands (SMG) with or without sublingual glands (SLG) from mouse embryos Following an approved IACUC protocol, remove E16 embryos from 2 timed pregnant females of the desired genotype.

    Techniques: Incubation, Sedimentation, Imaging, Staining